Using de novo sequencing to obtain the genomic information of microbes provides a fresh start for exploring the genetic structure and functions, studying the evolutionary origin of microbial populations, as well as developing potential applications of these abundant microbes in medicine, disease, agriculture, and environment.

For the microbial genome, Genetikode offers de novo sequencing service using Illumina platforms. We provide multifaceted sequencing services including genome survey, frame map, complete map, and fine map tailored to different research needs. For each project, our scientists will design the best sequencing strategy utilizing an optimal combination of short reads and long-range sequencing information to achieve the most comprehensive de novo assembly results for your genome of interest.

• DNA amount for survey: ≥ 10 µg.
• DNA amount for genome de novo sequencing per library: ≥ 2 µg (for Illumina sequencing) and > 20 µg (for PacBio sequencing).
• DNA concentration: ≥ 50 ng/µL (for Illumina sequencing) and > 80 ng/µL (for PacBio sequencing).
• Purity: No degradation, no DNA contamination.